Prediction of coronary heart disease incidence in a general male population by circulating non-coding small RNA sRNY1-5p in a nested case–control study

Vera L Costa (1) , Jean-Bernard Ruidavets (2) , Vanina Bongard (2, 3) , Bertrand Perret (4, 5) , Emanuela Repetto (1) , Maria G Stathopoulou (1) , Fabrizio Serra (1) , Mohamed Benahmed (1) , Claire Mauduit (1) , Valerie Grandjean (1) , Jean Ferrières (2, 3) , Laurent O Martinez (4, 2) , Michele Trabucchi (1)

1 C3M - Centre méditerranéen de médecine moléculaire
2 LEASP - Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps
3 Fédération de Cardiologie [CHU Toulouse]
4 UPS/Inserm U1297 - I2MC - Institut des Maladies Métaboliques et Casdiovasculaires
5 Laboratoire de Biochimie [CHU Toulouse]

During the development of atherosclerotic lesion, s-RNYs (small RNAs of about 24/34 nucleotides) are derived by the processing of long Ro-associated non-coding RNAs (RNYs) in macrophages. The levels of serum s-RNYs have been found significantly upregulated in patients with coronary heart disease (CHD) compared to age-matched CHD-free individuals. The present study aimed to examine the predictive value of serum s-RNYs for CHD events in the general male population. Within the frame of nested-case-control study, the GENES study, we measured the absolute expression of a RNY-derived small RNA, the s-RNY1-5p, in the serum of individuals (without CHD at baseline) who encountered a CHD event within 12 years of follow-up (n = 30) (Cases) and compared them to individuals who remained event-free (Controls) (n = 30). The expression of s-RNY1-5p in serum was significantly upregulated in Cases compared to Controls (p = 0.027). The proportion of CHD event-free was significantly higher among individuals with serum s-RNY1-5p below the median value (631 molecules/mL). In a multivariable model adjusted for age, smoking, hypertension, diabetes and dyslipidemia, the risk of CHD events increased more than fourfold in individuals with serum s-RNY1-5p above the median value (HR, 4.36; 95% CI 1.22-15.60). A positive association with CHD events was also observed when considering s-RNY1-5p as a continuous variable (p = 0.022). Based on our results, we conclude that serum s-RNY1-5p is an independent predictor of CHD events in a general male population and might be a relevant biomarker for early detection of cardiovascular diseases.

Domaines: Cardiologie et système cardiovasculaire; Santé publique et épidémiologie

Vera L Costa, Jean-Bernard Ruidavets, Vanina Bongard, Bertrand Perret, Emanuela Repetto, et al.. Prediction of coronary heart disease incidence in a general male population by circulating non-coding small RNA sRNY1-5p in a nested case–control study. Scientific Reports, 2021, 11 (1), pp.1837. ⟨10.1038/s41598-021-81221-8⟩. ⟨hal-04490999⟩